Burkholderia pseudomallei K96243, BPSL3073 (prfA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL3073
Name
prfA
Replicon chromosome 1
Genomic location 3667081 - 3668163 (+ strand)

Cross-References

RefSeq YP_109668.1
GI 53720682
Entrez 3092221
GI 53720682
INSDC CAH37084.1
NCBI Locus Tag BPSL3073
RefSeq YP_109668.1,GeneID
UniParc UPI000043B5FB
UniProtKB Acc Q63QF0
UniProtKB ID RF1_BURPS
UniRef100 UniRef100_A3NZS4
UniRef50 UniRef50_Q2KZZ3
UniRef90 UniRef90_Q2SUG2

Product

Feature Type CDS
Coding Frame 1
Product Name
peptide chain release factor 1
Synonyms
Evidence for Translation
Charge (pH 7) -8.39
Kyte-Doolittle Hydrophobicity Value -0.610
Molecular Weight (kDa) 40484.1
Isoelectric Point (pI) 5.65

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Extracellular Class 1
quantitative mass spectrometry evidence
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 593 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000399 (654 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL3073
Search term: prfA

Human Homologs

Ensembl 97, assembly GRCh38.p12
mitochondrial translational release factor 1 like [Source:HGNC Symbol;Acc:HGNC:21051]
E-value: 2.5e-70
Percent Identity: 44.4

References

Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268