Burkholderia pseudomallei K96243, BPSL3041 (pacL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
Locus Tag
BPSL3041
Name
pacL
Synonym: paaZ
Synonym: putative 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase
Replicon chromosome 1
Genomic location 3630348 - 3632054 (+ strand)
CommentIn E. coli, paaZ encodes a bifunctional protein consisting of an N-terminal NADP-dependent aldehyde dehydrogenase domain and a C-terminal MaoC-like (R)-specific enoyl-CoA hydratase domain. In Burkholderia genomes, a gene incorrectly annotated as paaZ (e.g WQ49_RS11500) encodes only one of the two domains found in E.coli paaZ (the domain with semialdehyde dehydrogenase activity) and so is renamed PacL (PMID:21296885) but was also referred to as PaaZ-ALDH in the manuscript. In the same study , a separate A. aromaticum, protein (UniProtKB:Q5P232) with the (R)-hydratase activity was annotated as Ech-Aa and the Burkholderia ortholog was annotated as PaaZ-ECH.

Cross-References

RefSeq YP_109636.1
GI 53720650
Entrez 3092152
GI 53720650
INSDC CAH37052.1
NCBI Locus Tag BPSL3041
RefSeq YP_109636.1,GeneID
UniParc UPI000043B5DB
UniProtKB Acc Q63QI2
UniProtKB ID Q63QI2_BURPS
UniRef100 UniRef100_Q63QI2
UniRef50 UniRef50_A1K261
UniRef90 UniRef90_Q2SUJ2

Product

Feature Type CDS
Coding Frame 1
Product Name
PacL
Product Name Confidence: Class 2
Synonyms phenylacetic acid degradation oxidoreductase
Evidence for Translation
Charge (pH 7) -5.77
Kyte-Doolittle Hydrophobicity Value -0.090
Molecular Weight (kDa) 60603.9
Isoelectric Point (pI) 6.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
quantitative mass spectrometry evidence
Cytoplasmic Class 3
Extracellular Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 476 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000433 (655 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL3041
Search term: pacL
Search term: PacL

References

Quantitative Proteomic Analysis of Burkholderia pseudomallei Bsa Type III Secretion System Effectors Using Hypersecreting Mutants.
Vander Broek CW(1), Chalmers KJ(2), Stevens MP(1), Stevens JM(3).
Mol Cell Proteomics. 2015 Apr;14(4):905-16.
PubMed ID: 25635268
PubMed ID: 21296885
A functional phenylacetic acid catabolic pathway is required for full pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans host model.
Law RJ(1), Hamlin JN, Sivro A, McCorrister SJ, Cardama GA, Cardona ST.
J Bacteriol. 2008 Nov;190(21):7209-18.
PubMed ID: 18776009