|Strain||Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1|
Synonym: putative 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase
|Genomic location||3630348 - 3632054 (+ strand)|
|NCBI Locus Tag||BPSL3041|
phenylacetic acid degradation oxidoreductase
|Comment||In E. coli, paaZ encodes a bifunctional protein consisting of an N-terminal NADP-dependent aldehyde dehydrogenase domain and a C-terminal MaoC-like (R)-specific enoyl-CoA hydratase domain. In Burkholderia genomes, a gene incorrectly annotated as paaZ (e.g WQ49_RS11500) encodes only one of the two domains found in E.coli paaZ (the domain with semialdehyde dehydrogenase activity) and so is renamed PacL (PMID:21296885) but was also referred to as PaaZ-ALDH in the manuscript. In the same study , a separate A. aromaticum, protein (UniProtKB:Q5P232) with the (R)-hydratase activity was annotated as Ech-Aa and the Burkholderia ortholog was annotated as PaaZ-ECH.|
|Evidence for Translation|
|Charge (pH 7)||-5.77|
|Kyte-Doolittle Hydrophobicity Value||-0.090|
|Molecular Weight (kDa)||60603.9|
|Isoelectric Point (pI)||6.28|
|Additional evidence for subcellular localization|
Found in both pathogen and nonpathogenic strainsHits to this gene were found in 476 genera
|Burkholderia Ortholog Group||
BG000433 (655 members)
|Putative Inparalogs||None Found|
|STRING database||Search for predicted protein-protein interactions using:
Search term: BPSL3041
Search term: pacL
Search term: PacL
Quantitative Proteomic Analysis of Burkholderia pseudomallei Bsa Type III Secretion System Effectors Using Hypersecreting Mutants.Vander Broek CW(1), Chalmers KJ(2), Stevens MP(1), Stevens JM(3).
Mol Cell Proteomics. 2015 Apr;14(4):905-16.
PubMed ID: 25635268
PubMed ID: 21296885
A functional phenylacetic acid catabolic pathway is required for full pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans host model.Law RJ(1), Hamlin JN, Sivro A, McCorrister SJ, Cardama GA, Cardona ST.
J Bacteriol. 2008 Nov;190(21):7209-18.
PubMed ID: 18776009