Burkholderia pseudomallei K96243, BPSL2953 (tktA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL2953
Name
tktA
Replicon chromosome 1
Genomic location 3524452 - 3526479 (- strand)

Cross-References

RefSeq YP_109547.1
GI 53720561
Entrez 3094803
GI 53720561
NCBI Locus Tag BPSL2953
RefSeq YP_109547.1,GeneID
UniProtKB Acc Q63QS1
UniProtKB ID Q63QS1_BURPS

Product

Feature Type CDS
Coding Frame 1
Product Name
transketolase
Synonyms
Evidence for Translation
Charge (pH 7) -7.63
Kyte-Doolittle Hydrophobicity Value -0.128
Molecular Weight (kDa) 72693.7
Isoelectric Point (pI) 6.48

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Cytoplasmic Class 3
Extracellular Class 1
quantitative mass spectrometry evidence
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3UPT TRANSFERASE 11/18/11 Crystal structure of a transketolase from Burkholderia pseudomallei bound to TPP, calcium and ribose-5-phosphate BURKHOLDERIA THAILANDENSIS 2.4 X-RAY DIFFRACTION 96.1
3UK1 TRANSFERASE 11/08/11 Crystal structure of a transketolase from Burkholderia thailandensis with an oxidized cysteinesulfonic acid in the active site BURKHOLDERIA THAILANDENSIS 2.15 X-RAY DIFFRACTION 96.1

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 610 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000522 (655 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL2953
Search term: tktA
Search term: transketolase

Human Homologs

References

Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268