Burkholderia pseudomallei K96243, BPSL1518 (hfq)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Burkholderia pseudomallei K96243 - Assembly GCF_000011545.1
GCF_000011545.1|latest
Locus Tag
BPSL1518
Name
hfq
Replicon chromosome 1
Genomic location 1757798 - 1758037 (+ strand)

Cross-References

RefSeq YP_108138.1
GI 53719152
Entrez 3092453
GI 53719152
INSDC CAH35519.1
NCBI Locus Tag BPSL1518
RefSeq YP_108138.1,GeneID
UniParc UPI000043B02F
UniProtKB Acc Q63US8
UniProtKB ID HFQ_BURPS
UniRef100 UniRef100_A3MK69
UniRef50 UniRef50_Q0C0N8
UniRef90 UniRef90_A3MK69

Product

Feature Type CDS
Coding Frame 1
Product Name
RNA-binding protein Hfq
Synonyms
Evidence for Translation
Detected in B. pseudomallei strain DD503 using trypsin shaving and mass spectrometry (PMID:21391724).
Charge (pH 7) 1.70
Kyte-Doolittle Hydrophobicity Value -0.051
Molecular Weight (kDa) 8833.1
Isoelectric Point (pI) 9.01

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Extracellular Class 1
quantitative mass spectrometry evidence
Additional evidence for subcellular localization
Detected in Triton-insoluble fraction of lysozyme-treated B. pseudomallei DD503 cells using trypsin shaving and mass spectrometry (PMID:21391724).

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3SB2 CHAPERONE 06/03/11 Crystal Structure of the RNA chaperone Hfq from Herbaspirillum seropedicae SMR1 HERBASPIRILLUM SEROPEDICAE 2.6301 X-RAY DIFFRACTION 95.7

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 282 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG001644 (652 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BPSL1518
Search term: hfq

Human Homologs

References

Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
Vander Broek CW, Chalmers KJ, Stevens MP, Stevens JM
Mol Cell Proteomics 2015 Apr;14(4):905-16
PubMed ID: 25635268