Burkholderia cenocepacia K56-2Valvano, BURCENK562V_RS24215 (rpoN)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Feature Overview

Strain Burkholderia cenocepacia K56-2Valvano
Locus Tag
BURCENK562V_RS24215
NCBI Old Locus TagBURCENK562V_C1709
Name
rpoN
Replicon ctg7180000002938
Genomic location 412568 - 413959 (- strand)

Cross-References

RefSeq WP_006483492.1
GI 493529477
GI 493529477
NCBI Locus Tag BURCENK562V_RS24215
NCBI Old Locus Tag BURCENK562V_C1709
RefSeq WP_006483492.1

Product

Feature Type CDS
Coding Frame 1
Product Name
alternative sigma factor RpoN
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -10.21
Kyte-Doolittle Hydrophobicity Value -0.155
Molecular Weight (kDa) 51487.1
Isoelectric Point (pI) 5.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database BGD
Category Alternative Sigma factors
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes rpoN mutant cannot delay phagolysosomal fusion in mouse RAW 264.7 macrophages
Reference 18195023
Source 2
Database BGD
Category Alternative Sigma factors
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes rpoN mutant attenuated for virulence in agar bead model of chronic lung infection
Reference 15213146

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG001356 (665 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: rpoN

References

Identification of Burkholderia cenocepacia genes required for bacterial survival in vivo.
Hunt TA(1), Kooi C, Sokol PA, Valvano MA.
Infect Immun. 2004 Jul;72(7):4010-22.
PubMed ID: 15213146
Burkholderia cenocepacia requires the RpoN sigma factor for biofilm formation and intracellular trafficking within macrophages.
Saldías MS, Lamothe J, Wu R, Valvano MA.
Infect Immun. 2008 Mar;76(3):1059-67.
PubMed ID: 18195023