Burkholderia cenocepacia J2315, BCAL0408 (pacL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse

Gene Feature Overview

Strain Burkholderia cenocepacia J2315
Locus Tag
BCAL0408
NCBI Locus TagQU43_RS38575
Name
pacL
Synonym: putative 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase
Synonym: paaZ
Replicon chromosome 1
Genomic location 441343 - 443049 (- strand)
CommentIn E. coli, paaZ encodes a bifunctional protein consisting of an N-terminal NADP-dependent aldehyde dehydrogenase domain and a C-terminal MaoC-like (R)-specific enoyl-CoA hydratase domain. In Burkholderia genomes, a gene incorrectly annotated as paaZ (e.g WQ49_RS11500) encodes only one of the two domains found in E.coli paaZ (the domain with semialdehyde dehydrogenase activity) and so is renamed PacL (PMID:21296885) but was also referred to as PaaZ-ALDH in the manuscript. In the same study , a separate A. aromaticum, protein (UniProtKB:Q5P232) with the (R)-hydratase activity was annotated as Ech-Aa and the Burkholderia ortholog was annotated as PaaZ-ECH.

Cross-References

RefSeq WP_006491101.1
GI 206558814
Entrez 6933932
GI 206558814
INSDC CAR50719.1
NCBI Locus Tag QU43_RS38575
NCBI Old Locus Tag BCAL0408
RefSeq WP_006491101.1,GeneID
UniParc UPI00017B8ED6
UniProtKB Acc B4E7B9
UniProtKB ID B4E7B9_BURCJ
UniRef100 UniRef100_A0A095EUV7
UniRef50 UniRef50_A1K261
UniRef90 UniRef90_G7H919

Product

Feature Type CDS
Coding Frame 1
Product Name
PacL
Product Name Confidence: Class 2
Synonyms putative phenylacetic acid degradation oxidoreductase
Evidence for Translation
Charge (pH 7) -9.98
Kyte-Doolittle Hydrophobicity Value -0.051
Molecular Weight (kDa) 60502.6
Isoelectric Point (pI) 5.94

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5UJU OXIDOREDUCTASE 01/19/17 Crystal structure of NAD-dependent aldehyde dehydrogenase from Burkholderia multivorans BURKHOLDERIA MULTIVORANS (STRAIN ATCC 17616 / 249) 2.05 X-RAY DIFFRACTION 94.9

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 504 genera

Orthologs/Comparative Genomics

Burkholderia Ortholog Group BG000433 (655 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: BCAL0408
Search term: pacL
Search term: PacL

References

A functional phenylacetic acid catabolic pathway is required for full pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans host model.
Law RJ(1), Hamlin JN, Sivro A, McCorrister SJ, Cardama GA, Cardona ST.
J Bacteriol. 2008 Nov;190(21):7209-18.
PubMed ID: 18776009
PubMed ID: 21296885