Burkholderia dolosa AU0158, AK34_RS25965 (pacL)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Burkholderia dolosa AU0158 (complete genome)
GCF_000959505.1|latest |
| Locus Tag |
AK34_RS25965
|
| NCBI Old Locus Tag | AK34_2604 |
| Name |
pacL
Synonym: putative 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase Synonym: BDAG_02607 |
| Replicon | chromosome 1 |
| Genomic location | 2798931 - 2800637 (+ strand) |
| Comment | In E. coli, paaZ encodes a bifunctional protein consisting of an N-terminal NADP-dependent aldehyde dehydrogenase domain and a C-terminal MaoC-like (R)-specific enoyl-CoA hydratase domain. In Burkholderia genomes, a gene incorrectly annotated as paaZ (e.g WQ49_RS11500) encodes only one of the two domains found in E.coli paaZ (the domain with semialdehyde dehydrogenase activity) and so is renamed PacL (PMID:21296885) but was also referred to as PaaZ-ALDH in the manuscript. In the same study , a separate A. aromaticum, protein (UniProtKB:Q5P232) with the (R)-hydratase activity was annotated as Ech-Aa and the Burkholderia ortholog was annotated as PaaZ-ECH. |
Cross-References
| RefSeq | WP_006765272.1 |
| GI | 493817705 |
| NCBI Locus Tag | AK34_RS25965 |
| NCBI Old Locus Tag | AK34_2604 |
| RefSeq | WP_006765272.1 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
PacL
|
| Synonyms |
aldehyde dehydrogenase |
| Evidence for Translation | |
| Charge (pH 7) | -6.23 |
| Kyte-Doolittle Hydrophobicity Value | -0.042 |
| Molecular Weight (kDa) | 60447.7 |
| Isoelectric Point (pI) | 6.31 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold 2 Protein Structure Predictions
Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 5UJU | OXIDOREDUCTASE | 01/19/17 | Crystal structure of NAD-dependent aldehyde dehydrogenase from Burkholderia multivorans | BURKHOLDERIA MULTIVORANS (STRAIN ATCC 17616 / 249) | 2.05 | X-RAY DIFFRACTION | 93.7 |
Pathogen Association Analysis
| Results | Analysis not done |
Orthologs/Comparative Genomics
| Burkholderia Ortholog Group |
BG000433 (655 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: AK34_RS25965
Search term: pacL
Search term: PacL
|
Human Homologs
References
|
Studies on the mechanism of ring hydrolysis in phenylacetate degradation: a metabolic branching point.
Teufel R, Gantert C, Voss M, Eisenreich W, Haehnel W, Fuchs G
J Biol Chem 2011 Apr 1;286(13):11021-34
PubMed ID: 21296885
|
|
A functional phenylacetic acid catabolic pathway is required for full pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans host model.
Law RJ, Hamlin JN, Sivro A, McCorrister SJ, Cardama GA, Cardona ST
J Bacteriol 2008 Nov;190(21):7209-18
PubMed ID: 18776009
|